The permit holder is allowed to add the following changes to the permit merely by notifying them:

1. a change in the referral to one or more notices or permits for restricted use;
2. a change in the composition of the DNA preparation, with the exception of an addition of nucleic acid, viruses or micro-organisms;
3. an expansion of the number of test subjects, as long as the total number does not exceed the maximum number allowed under the permit;
4. a change in the starting date of the research that is conducted on the release, or in its end date, as long as this does not lead to an exceedance of the end data in the original permit; 

Notifications that have no consequences for the environmental risk assessment are specified in Article 35 of the GMO Regulation 2013 (in Dutch). Click here for an explanation of Article 35.

In principle, the permit holder is allowed to add any changes in the work to the permit merely by notifying them, provided that these changes remain within the set framework of the environmental risk assessment. All changes in the permit can be notified, bar the following exceptions:

1. the addition of another location;
2. if the changes concern an expansion in the number of test subjects that leads to an exceedance of the maximum number of test subjects allowed under the original permit;
3. if they concern a change in the end date of the research that leads to an exceedance of the end date in the original permit;
4. if the issued permit relates to gene therapy in humans and the changes are not limited to gene therapy in humans;
5. if the changes concern an adjustment in the administering method that does not remain limited to tattooing or direct injection into the skin of striated muscle tissue;
6. if the changes concern the addition of a viral sequence other than any of the excepted viral sequences;
7. if the changes concern the addition of antibiotic-resistant genes, unless these confer resistance solely to kanamycin or neomycin.

The excepted changes may mean that the permit must be adjusted, or that a new permit must be applied for.

The permit holder is allowed to add a number of changes to the permit merely by notifying them. The following changes in the permit may be reported:

1. a change in the referral to one or more notices or permits for restricted use;
2. a change in the composition of the DNA preparation, with the exception of an addition of nucleic acid, viruses or micro-organisms, excluding the changes to the naked DNA as described under points 6 to 9;
3. a change in the production of the DNA preparation, as long as the end product will not be rejected on the grounds of the rejection criteria that apply;
4. an expansion of the number of test subjects (animal or human), as long as the total number does not exceed the maximum number allowed under the permit;
5. a change in the starting date of the research that is conducted on the release, or in its end date, as long as this does not lead to an exceedance of the end data in the original permit;
6. replacement of the existing promoter by a non-viral or non-bacterial promoter;
7. removal from the DNA of sequences coding for antibiotic resistance;
8. replacement of the existing origin of replication (ori) by an ori of the plasmids pBR322, pUC (ColE1 ori), or p15A (pACYC series of plasmids);
9. replacement of the existing antibiotic-resistance gene by a gene that, in prokaryotes, is resistant only to kanamycin or neomycin.